Access to the complete content on Oxford Reference requires a subscription or purchase. This standard calcium solution of known concentration is used to determine the exact concentration of the EDTA solution. 1252018 To prepare 1000 mL of your standard solution the solution you will use for Trial 1 quantitatively transfer 100 mL of the stock salicylic acid solution you prepared in Step 1 to a 100 mL volumetric flask.